These merchandise contain random primers and oligo (dT) primers for initial strand cDNA synthesis, gene specific primers for sequencing, ahead and reverse sequencing primers and kits, along with the MegaPlex RT Primers and Megaplex primer swimming pools produced for evaluating expression profiles.
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Pools of pre-intended primers can help generate in depth expression profiles, Specially where by assay sensitivity is critically essential.
Assist with this selection on, the program will instantly retrieve the SNP data contained in template (making use of GenBank accession or GI as template is required) and steer clear of deciding upon primers throughout the SNP areas. Repeat filter
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You can even specify while in the fields down below the nominal variety of nucleotides that the still left or the best primer will need to have on possibly side with the junctions. This selection is useful If you prefer a primer to your span specific junction around the template. Notice that this feature can't be Utilized in association While using the "Exon/intron range" solutions above. 5' facet overlaps
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Swimming check here pools of pre-built primers can assist generate comprehensive expression profiles, Specifically where assay sensitivity is critically crucial.
Not like oligo(dT) primers, these primers are not template particular and therefore here are suited to DNA synthesis applying Klenow fragments with DNA templates or for cDNA synthesis using reverse transcriptase with mRNA templates.
Pools of pre-created primers can help generate detailed expression profiles, Specially in which assay sensitivity is critically vital.
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